Recently Published Papers
Original Research Article
Selection of Detergents Suitable for IBMR3 (Mab) using Balb/c Mouse Muscle
Qutaiba K. J. Alrawi, Nada, S. Alzubydy
DOI : 10.36344/ccijmb.2021.v03i08.002 | Cross Current Int J Med Biosci | 2021 | 3(8) | 75-82 |
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Monoclonal antibodies (Mab) and their fragments have been widely used for diagnostic and therapeutic purposes. Monoclonal antibodies IBMR3 hybridoma cells were produced in a previous study. In my study I used four types of detergents to fine the more suitable as the best Lysis buffer for monoclonal anti bodies using Balb/ c mouse tissue muscle. The four detergents includes; NP- 40, Igepal, Chaps and Triton X-100. Detergents were used in the laboratory to solubilize biological macromolecules such as proteins. These are none denaturing solvents; they also increase emulsification and solubilization, act as solubilize membrane proteins in their native state. The mouse samples were lysed in different lysis buffer detergents, the extracting protein where subjected on the SDS-PAGE electrophoresis, the separated protein bands were transferred to PVDF/ Polyvinylidene difluoride membrane for Immunoblotting technique. The immunblot were subsequently subjected to densitometry analysis to get the value of molecular weight, peak height and raw volume of the protein band. The results of muscle protein concentration of Blab/c mouse after using standard methods were shown (NP-40, 3.214 μg / μl), (Igepal, 3.647 μg / μl), (CHAPS, 3.925 μg / μl and Triton X-100, 4.214 μg / μl). The highest concentration of the muscle protein was obtained from using Triton X-100, followed by CHAPS, then by Igepal and in NP- 40.
Original Research Article
Genetic Variability, Heritability and Genetic Advance of Ten Sesame (Sesamum indicum L.) varieties at Omo Kuraz, Southern Ethiopia
Enaniye Tigabu, Feven Million, Afework Asfaw
DOI : 10.36344/ccijavs.2021.v03i07.001 | Cross Current Int J Agri Vet Sci; 2021, 3(7): 60-65
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Ten sesame varieties were evaluated using Randomized complete block design with three replications at Omo kuraz -1 sugar development project of South Omo, Southern Ethiopia, during 2018/19 under irrigated condition to estimate phenotypic and genotypic coefficients of variation, heritability and genetic advance for some important characters in sesame (Sesamum indicum L.). The treatment consisted of ten varieties of sesame namely: E, Tate, Kelafo- 74, Mehando-80, T-85, Adi, Abasena, S, Argene and Serkamo. Data were collected on phenological, growth, yield, yield components, oil content and oil yield and analyzed using SAS software version 9.0. The results showed that there were significant differences among the varieties for all characters studied. High estimates for GCV and PCV were observed in seed yield and oil yield, While Plant height to first branch, Plant height to first capsule, number of capsules per plant and harvest index showed moderate PCV and GCV and the remaining traits recorded low PCV and GCV. High estimates for heritability values were observed for traits Seed yield, Plant height to first branch, TSW, Plant height to first capsule, internode length and harvest index which indicated that for breeding activities and can be utilized in subsequent selections based on phenotypic expressions of individual plants for specific traits. And it further indicates the limited influence of the environment on the phenotypic expressions of the traits. Further computation for GAM indicated that high estimates were found for Seed yield, number of capsules per plant, Harvest index, Plant height to first branch, Plant height to first capsule which could further confirm the easy of selections based on phenotypic traits after cycle of selection using 5 % selection intensity. This study generally indicated that there were significance genetic variations among the varieties. Thus, there is enormous opportunity of using the germplasm in the improvement program for ..........
Original Research Article
Digitalization and Organizational Performance of Logistics Firms in Ifako-Ijaye Local Government of Lagos State
Udemba Ujunwa Chinelo
DOI : 10.36344/ccijemms.2021.v03i06.001 | Cross Current Int J Econ Manag Media Stud; 2021; 3(6): 62-70
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This study examined the impact of digitalization on organizational performance in Logistics firms in Ifako-Ijaye LGA of Lagos State. Five research questions and two hypotheses guided the study. In line with the objective of the study, descriptive research design was adopted. The population of the study was staff of 108 logistics firms in Ifako-Ijaye LGA of Lagos State. A sample size of 44 respondents were drawn from the entire population using purposive sampling techniques. The instruments for data collection were a researcher's self-developed questionnaire titled Digitalization and Organizational Performance in Logistics Firms (DOPLF) and a researcher-structured interview. The research questions were answered using mean and standard deviation while the null hypotheses were tested at 0.05 level of significance using the t-test statistics. Findings revealed that respondents strongly agreed on how the adoption of digital transformation has changed the logistics businesses as well as on the extent to which digital technologies and connectivity impact organizational productivity. It was concluded that digitalization to a high extent impacts organization performance and has the capability to generate huge profits when ICT tools are successfully implemented. The study hence recommended the following amongst others that; logistics companies should embrace IT tools and services to gain a competitive advantage and improve service delivery to customers, have more self-service enabled services, and automate all critical processes to improve efficiency, reliability, and control in the organization.
Original Research Article
Influence of Family Size on Social Adjustment of Learners with Visual Impairment in Akwa Ibom State
Dr. Nwachukwu, Kingsley Ezechinyere, Archibong, I. Etim
DOI : 10.36344/ccijhss.2021.v07i10.002 | Cross Current Int J Peer Reviewed J Human Soc Sci | 2021 | 7(10) | 182-186 |
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This study investigated on influence of family size on social adjustment of learners with visual impairment in Akwa Ibom State. Three null hypotheses were postulated to guide the study. The study adopted descriptive survey research design. The population of the study comprised all the 79 learners with visual impairment in all the three Special Education Centres in AkwaIbom State. A sample size of 79 learners with visual impairment selected through purposive sampling technique. The instrument used for data collection was Family Size and Social Adjustment Questionnaire (FSSAQ)” This was personally administered by the researcher with the help of teachers who served as research assistants. z-test was used to test the hypotheses at 0.05 level of significance. The study revealed that there is a significant influence of large family size and small family size on social adjustment of learners with visual impairment. Also, there is no significant influence of gender on social adjustment of learners with visual impairment in Akwa Ibom State. Based on the findings of the study, it was recommended among others that the first responsibility of the parents and teachers should be to know the areas where the students lack adjustment and try to give better conditions for proper adjustment in every sphere.
Belgian Unforgotten Historical Evils Orchestrated Against the Congolese (BUHEOAC) till the Assassination of Famous Black African Nationalists (AFBAN) 19th-20th Centuries
Dr. Njuafac Kenedy Fonju
DOI : 10.36344/ccijhss.2021.v07i10.001 | Cross Current Int J Peer Reviewed J Human Soc Sci | 2021 | 7(10) | 164-181 |
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A deeper historical study and understanding of Belgian BUHEOAC and AFBAN in the African Continent were manifested through 28 different Diplomatic Agents with distinguished administrative portfolios beginning with Francis de Winton as an Administrator General (A.G) from 22 April 1884 to Hendrik Cornelis as Governor General (G.G.) whose massacred era came to an end on 30th June 1960.Then, On 1st July 1960, the former Congo Free State (CFS) later Belgian Congo (B.C) became an independent country bearing the name the Republic of the Congo (R.C) with the worst and horrible homicide with total conspiracy masterminded by the Western Capitalist World following the assassination of Patrice Lumumba who was qualified as a pro-Communist African Nationalist during the second half of the 20th Century. Those cannibalistic activities occurred with total complicities of the 7 Belgian Monarchical men alongside with 53 Prime Ministers identified in this study. The activities of the Belgian colonial agents are comparable to the aggressive actions of Adolf Hitler and Benito Mussolini in Africa and elsewhere around the World during the first half of the 20th Century as aggressors and Statesmen who acquired honours from human massacred. Therefore, what are the Belgian Government and Kings thinking of the unforgotten evils of their ancestors against the Congolese? The scrutiny of, diverse sources and documentaries enable us to use a historical analytical approach with statistical tables of the actors involved between 1884 and 1960 as a modest contribution to the 21st Century constant reawaking of reparation requested by the country’s siblings.
