Evaluation of Cytotoxic Potentials of Microalgae on Hela, Hepg-2, HT-29 and EACC Cells to Develop Anticancer Agents
DOI : 10.36344/ccijemms.2020.v02i02.004 | Cross Current Int J Med Biosci, 2019; 2(2):11-16.
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Finding more effective methods to treat cancer remains a challenge, and development of new therapeutic agents for cancer treatment is essential for continued progress against the disease. This study was conducted to evaluate the in vitro antiproliferative activity of methanolic extracts of Chlorella vulgaris and Nostoc muscorum against Human cervical Cancer cells (HeLa), liver hepatocellular cells (HepG-2), Human colorectal adenocarcinoma cells (HT-29) and Ehrlich Ascites Carcinoma Cells (EACC) in order to determine the bioacitivities of microalgae. Morphological changes of cancer cells treated with the selected extract were observed by phase contrast microscopy. The cells were incubated with increasing concentrations of microalgal extracts for 24, 48, and 72 h, and cell viability was determined by the Sulforhodamine B (SRB) assay. After treatment of cancerous cells with microalgal extract for 48 h, 55% of the cells were viable, 38% were in early apoptosis, 5% were in late apoptosis, and 1% exhibited necrosis. Further studies are needed to discover the mechanisms underlying the antiproliferative activity of microalgal extracts against cancer cell lines and to identify specific bioactive compounds that are responsible for the observed activities.
Thrombectomy for Acute Treatment of Trombosed Hemorrhoids
Alp Yildiz, Mete Keskin, Aybala Yildiz and Veysel Baris Turhan
DOI : 10.36344/CCIJMB.2020.v02i01.003 | Cross Current Int J Med Biosci, 2019; 2(1):8-10.
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Hemorrhoidal disease is a common anorectal disorder characterized by the symptomatic enlargement and distal displacement of the hemorrhoids, which are the normal anal cushions. The main symptoms of hemorrhoidal disease include rectal bleeding, prolapse, pain, thrombosis, mucus discharge, and pruritus. Hemorrhoids are classified based on their location and the degree of prolapse. Clinical management of hemorrhoidal disease is based on severity. While conservative strategies are used for Patients with grades I or II disease, radical surgery is recommended for patients with grades III or IV hemorrhoids. Hereby we present our results of thrombectomy on acute treatment in patients with acute thrombosed hemorrhoids. 54 consecutive cases included this study. The mean age of the Patients was 38.5(+/-4.1), 22 patients were male, 32 patients were female. All patients underwent thrombectomy procedure and 8th hour 12th hour, 24th hour and 3rd day VAS scores were (4.1; 3.4; 5.8; 2.1) consecutively. 1 patient had developed intraoperative bleeding which controlled with monopolar electrocautery application, no postoperative bleeding has seen. 1 patient developed slight infection which controlled with simple antibiotics. No other complication has seen after procedure. Our study’s the results were similar with literature and as conclusion we believe thrombectomy is a very safe and effective method of management of acute hemorrhoidal crisis in patients with thrombosed hemorrhoids.
Limberg Flap Method for Pilonidal Sinus Disease
Alp Yildiz, Mete Keskin, Aybala Yildiz and Veysel Baris Turhan
DOI : 10.36344/CCIJMB.2020.v02i01.002 | Cross Current Int J Med Biosci, 2019; 2(1): 6-7.
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Sacrococcygeal pilonidal sinus disease is a common chronic inflammation of the natal cleft and is mainly seen in young adults. Authors of some earlier works regarded the condition as congenital. Recently, it has become widely accepted that the condition is acquired, and thought to originate from hair follicles. Many treatment options has described in literature, thus in this study we present our results with Limberg flap reconstruction on pilonidal sinus disease. 38 patients underwent limberg flap reconstruction for pilonidal sinus disease enrolled in this study. No bleeding has occur intra and postoperatively, 2 patients developed urinary retention (%5.2), on 1 patient with diabetes mellitus wound dehiscence has developed which has treated with 3 week long simple debridement method (%2.6). 1 patient developed superficial infection treated with basic antibiotics (%2.6). No other complications has developed. No recurrence has seen in -mean -11.2 month period. We think limberg flap closure on pilonidal sinus disease is an effective and safe option.
Diagnosis of Chronic Obstructive Air Way Disease-Made Easy
Dr. Anil Batta
DOI : 10.36344/CCIJMB.2020.v02i01.001 | Cross Current Int J Med Biosci, 2019; 2(1): 1-5.
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Chronic obstructive pulmonary disease is an inflammatory disease. Together with oxidant stimuli, which directly affect lung structures, macrophages, neutrophils and CD8+ lymphocytes actively participate in the pathogenesis of the disease and promote biochemical reactions that result in progressive alteration of the upper airways and irreversible lung remodeling. The release of substances promoted by inflammatory cell recruitment and by oxidative stress lead to a temporary imbalance in the pulmonary defense mechanisms. Understanding the long-term maintenance of this imbalance is key to understanding the current physiopathology of the disease. The present study explores the cellular and molecular alterations seen in chronic obstructive pulmonary disease.
A Study on the Effect of Temperature in Effluent Treatment Process
DOI : 10.36344/CCIJMB.2019.v01i06.005 | Cross Current Int J Med Biosci, 2019; 1(6): 165-168.
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An experimental investigation was carried out to determine the suitable temperature for efficient functioning of Effluent treatment plant. For this purpose different temperatures were maintained to reduce the Chemical Oxygen Demand and Biological Oxygen Demand during anaerobic process. Experiments were carried out by maintaining the pH 6.8- 7.2 at laboratory level for 5 days. Different temperature ranges i.e. 250C, 300C, 350 C and 400 C were maintained throughout the experiments. Among these four temperatures 400 C had given good results in reducing the COD and BOD levels.
Bacteria Associated With the American Cockroach Periplanata Americana (Dictyoptera: Blatellidae) In Amassoma, Bayelsa State, Nigeria
Ukoroije, Rosemary Boate* and Bobmanuel Rosetta Bekinwari
DOI : 10.36344/CCIJMB.2019.v01i06.002 | Cross Current Int J Med Biosci, 2019; 1(6): 146-151.
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Cockroaches have become the most common peridomestic insect pest of public health and epidemiological importance. The presence raises safety concerns, especially as carriers of food-borne pathogens and food-spoilage organisms. Using a swab bacteriological technique, investigations were carried out on the wings, legs and mouth parts of cockroaches trapped from sewers, kitchens and bathrooms from household in Amassoma, Bayelsa State. The density of microorganisms by standard plate count was used to analyze the samples. Microbial load gotten were 62 x 103 _ 76 x 103 cfu/ml-1 for wings, 45 x 103 ¬–53 x 103 cfu/ml-1for legs and 36 x 103¬- 41 x103 cfu/ml-1 for mouthparts respectively regarding heterotrophic bacterial count and 59 x 103 - 62 x 103 cfu/ml-1, 41 x 103 ¬- 53 x 103 cfu/ml-1 and 36 x 103 ¬- 43 x 103 cfu/ml-1 for fecal coliform count respectively. Five bacteria genera of the family Enterobacteriaceae, known as Opportunistic pathogens and responsible for food spoilage were identified such as Escherichia coli (33.3%) most frequently isolated, Citrobacter specie (16.7%), Enterobacter specie (16.7%) while Pseudomonas specie (22.2%) and Klebseilla specie (11.1%) were the least isolated. Highest bacteria count was recorded from the wings followed by the legs and the mouth. Cockroaches can readily move from contaminated zones such as faeces to food preparation areas spreading food spoilage and disease-causing organisms onto the food. Proper care in disposal of food remnants and overall cleanliness at the households prevent cockroaches from foraging in the kitchen, bedroom and toilet
Cockroach Infestation and Their Perceived Importance in Yenagoa Metropolis of Bayelsa State, Nigeria
Ukoroije, Rosemary Boate* and Bobmanuel, Rosetta Bekinwari
DOI : 10.36344/CCIJMB.2019.v01i06.007 | Cross Current Int J Med Biosci, 2019; 1(6): 175-182.
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This study aimed at cockroach infestations and their perceived importance in disease transmission was carried out in Yenagoa metropolis of Bayelsa State of Nigeria using sample survey method involving the retrieval of 225 descriptive questionnaires from the people bound on their knowledge of cockroach as vector of pathogenic organisms and the possible modes of control. On the frequency of cockroach infestation in habitats, respondents reported 20.40% cockroach infestation in private homes, schools (7.80%), offices (5.90%), hospitals (12.20%), toilets (18.40%), dust bins (15.70%) and restaurants (19.60%). 82.4% of the respondents affirmed that cockroaches are carriers of disease pathogens and are implicated as causative organisms of allergies and respiratory disease. About 23.5% were well aware that cockroach infestation triggers asthmatic attack in sensitive people. All the surveyed houses reported cockroach infestation, with high prevalence in the older houses (71.40%) compared to the recently built ones (53.90%). 78.4% of the respondents gave poor sanitary condition as an indicator for high cockroach prevalence. Concerning best method of control, 54.90% voted for the use of synthetic pesticides, biological control (23.53%) and cultural control (21.57%). Therefore, the teeming populace of Bayelsa State should be educated as to the perceived importance of the prevalence of cockroaches in homes, hospitals and food preparation centers especially as vectors of pathogenic organisms and be further sensitized on the best control practices such as good sanitation practice, food hygiene, stocking foodstuffs in sealed containers and proper disposal of refuse so as to reduce cockroach related menace.